Fate-Mapping of Yolk Sac-Derived Macrophages
Fate-Mapping of Yolk Sac-Derived Macrophages
View/Date of Publication
29.08.2023Publisher
SpringerPublisher Location
New YorkMetadata
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Citable Link (Handle URI) 
https://hdl.handle.net/20.500.11811/11072
Abstract
To better understand the distinct functions of yolk-sac-derived tissue-resident macrophages (TRMs) and bone-marrow-derived macrophages in homeostasis and disease, it is important to trace the ontogeny of these cells. The majority of TRMs originate from erythro-myeloid progenitors (EMPs). EMPs develop into pre-macrophages (pMacs), which can be detected starting at embryonic developmental day (E)9.0, and which give rise to all TRM during early development. pMacs start expressing the gene Cx3cr1, allowing us to genetically target the early yolk-sac wave of pMacs and their progeny. Here, we describe the protocol for the identification of yolk sac-derived TRMs utilizing in utero labelling of the inducible fate mapping Cx3cr1CreERT; Rosa26LSL-eYFP mouse model.
Subjects
macrophages, fate-mapping
Classification (DDC)
570 Biowissenschaften, BiologiePublished in
Tissue-Resident Macrophages: Methods and Protocols, pp. 129-137First Publication
https://doi.org/10.1007/978-1-0716-3437-0_8Part of Series
Methods in Molecular Biology ; 2713Splichalova, Iva; Mass, Elvira: Fate-Mapping of Yolk Sac-Derived Macrophages. In: Mass, Elvira (Hrsg.): Tissue-Resident Macrophages: Methods and Protocols. New York: Springer, 2023. 129-137.
Online-Ausgabe in bonndoc: https://hdl.handle.net/20.500.11811/11072
Online-Ausgabe in bonndoc: https://hdl.handle.net/20.500.11811/11072
@inbook{handle:20.500.11811/11072,
author = {{Iva Splichalova} and {Elvira Mass}},
editor = {{Elvira Mass}},
title = {Fate-Mapping of Yolk Sac-Derived Macrophages},
publisher = {Springer},
year = 2023,
month = aug,
booktitle = {Tissue-Resident Macrophages: Methods and Protocols},
volume = 2713,
pages = 129--137,
note = {To better understand the distinct functions of yolk-sac-derived tissue-resident macrophages (TRMs) and bone-marrow-derived macrophages in homeostasis and disease, it is important to trace the ontogeny of these cells. The majority of TRMs originate from erythro-myeloid progenitors (EMPs). EMPs develop into pre-macrophages (pMacs), which can be detected starting at embryonic developmental day (E)9.0, and which give rise to all TRM during early development. pMacs start expressing the gene Cx3cr1, allowing us to genetically target the early yolk-sac wave of pMacs and their progeny. Here, we describe the protocol for the identification of yolk sac-derived TRMs utilizing in utero labelling of the inducible fate mapping Cx3cr1CreERT; Rosa26LSL-eYFP mouse model.},
url = {https://hdl.handle.net/20.500.11811/11072}
}
author = {{Iva Splichalova} and {Elvira Mass}},
editor = {{Elvira Mass}},
title = {Fate-Mapping of Yolk Sac-Derived Macrophages},
publisher = {Springer},
year = 2023,
month = aug,
booktitle = {Tissue-Resident Macrophages: Methods and Protocols},
volume = 2713,
pages = 129--137,
note = {To better understand the distinct functions of yolk-sac-derived tissue-resident macrophages (TRMs) and bone-marrow-derived macrophages in homeostasis and disease, it is important to trace the ontogeny of these cells. The majority of TRMs originate from erythro-myeloid progenitors (EMPs). EMPs develop into pre-macrophages (pMacs), which can be detected starting at embryonic developmental day (E)9.0, and which give rise to all TRM during early development. pMacs start expressing the gene Cx3cr1, allowing us to genetically target the early yolk-sac wave of pMacs and their progeny. Here, we describe the protocol for the identification of yolk sac-derived TRMs utilizing in utero labelling of the inducible fate mapping Cx3cr1CreERT; Rosa26LSL-eYFP mouse model.},
url = {https://hdl.handle.net/20.500.11811/11072}
}
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