Klaus, Alina: Early transcriptomic plasticity in barley (Hordeum vulgare L.) seminal roots in response to abiotic stress. - Bonn, 2024. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5-76090
@phdthesis{handle:20.500.11811/11540,
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5-76090,
doi: https://doi.org/10.48565/bonndoc-287,
author = {{Alina Klaus}},
title = {Early transcriptomic plasticity in barley (Hordeum vulgare L.) seminal roots in response to abiotic stress},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2024,
month = may,

note = {Water deficit and soil salinity negatively affect plant growth and thus pose major threats to agricultural production and food security. Already one of these factors results in economic damage, while a combination of both stressors often has detrimental effects leading to total yield loss. Due to global warming, extreme weather events like droughts are expected to increase in the future. Formerly rain-fed land will become dependent on irrigation, further enhancing soil salinization, leading to constant abiotic stress combinations. Thus, it is imperative to examine and understand the processes and mechanisms that shape whole-root and root zone-specific responses to these abiotic constraints to secure food productivity under unfavorable environmental conditions. In this study we first investigated the effects of water deficit (- 0.8 MPa), salinity (150 mM NaCl) and their combination on the transcriptome of whole seminal roots of barley seedlings after 6 h and 24 h of stress exposure. Then, we examined the effect of the same water deficit on the transcriptome of the individual root zones: root cap and meristem, elongation zone and differentiation zone after 6 h, 24 h and 48 h of treatment.
RNA-sequencing revealed hundreds of genes that are differentially regulated in response to either treatment by time or root zone combination. Interestingly, we observed a treatment-wise temporal conservation of responsive genes in the whole-root study, with hundreds of additional genes activated in the later time point. This was not confirmed in the root zone-wise approach. Instead, we identified different phases of modulation with varying extents. The combination of stresses affected a unique set of differentially expressed genes, that were not differentially expressed in either individual stress scenario and whose extend exceeded the sum of the individual applications. Root zone-wise, we also detected several unique sets of differentially expressed genes according to their time by zone combination. Overall, the elongation zone was the most affected root zone.
Grouping the identified differentially expressed genes into a biological context showed that processes and pathways were shared between different treatments and root zones, yet their modulation and the involved genes remained specific. In general, alterations to the redox system, regulation of transporters, post-translational modification by protein kinases, adaptations in the energy metabolism, cell wall reorganization and modulation of transcription factor activity shaped the plant responses to abiotic stresses in a treatment, time and root zone-specific manner.
The findings presented here provide new insights into complex, dynamic transcriptomic responses, emphasizing the importance of combinatorial stress research and considering spatial and temporal resolution.},

url = {https://hdl.handle.net/20.500.11811/11540}
}

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