Browsing by Author "Ahmadi, Shiva"
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Application of BioID to in vitro organelle and in vivo cell-type-specific proteomics
Ahmadi, Shiva (2020-09-17)A genetically modified biotin ligase BirA (Arg118Gly), termed BioID (BirA*), can be induced to add a reactive form of biotin to proximate proteins in the cytosol and nucleus. The biotinylated proteins can be purified and ...