Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors
Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors
View/Date of Publication
14.11.2019Publisher
LondonPublisher Location
Biochemical SocietyMetadata
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Citable Link (Handle URI) 
https://hdl.handle.net/20.500.11811/9771
Abstract
The second messenger 3´, 5´-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatiotemporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.
Subjects
Biochemical Techniques & Resources, biosensors, cAMP, optogenetics
Classification (DDC)
570 Biowissenschaften, Biologie 610 Medizin, GesundheitPublished in
Biochemical Society transactions, 2019. 47, 6, pp. 1733-1747First Publication
https://doi.org/10.1042/BST20190246Klausen, Christina; Kaiser, Fabian; Stüven, Birthe; Hansen, Jan N.; Wachten, Dagmar: Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors. In: Biochemical Society transactions. 2019. 47, 6, 1733-1747.
Online-Ausgabe in bonndoc: https://hdl.handle.net/20.500.11811/9771
Online-Ausgabe in bonndoc: https://hdl.handle.net/20.500.11811/9771
@article{handle:20.500.11811/9771,
author = {{Christina Klausen} and {Fabian Kaiser} and {Birthe Stüven} and {Jan N. Hansen} and {Dagmar Wachten}},
title = {Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors},
publisher = {London},
year = 2019,
month = nov,
journal = {Biochemical Society transactions},
volume = 2019. 47,
number = 6,
pages = 1733--1747,
note = {The second messenger 3´, 5´-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatiotemporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.},
url = {https://hdl.handle.net/20.500.11811/9771}
}
author = {{Christina Klausen} and {Fabian Kaiser} and {Birthe Stüven} and {Jan N. Hansen} and {Dagmar Wachten}},
title = {Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors},
publisher = {London},
year = 2019,
month = nov,
journal = {Biochemical Society transactions},
volume = 2019. 47,
number = 6,
pages = 1733--1747,
note = {The second messenger 3´, 5´-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatiotemporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.},
url = {https://hdl.handle.net/20.500.11811/9771}
}
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