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Seasonal variations of sperm freezability associated with testicular germinal epithelium changes in domestic (Ovis aries) and wild (Ovis musimon) rams

dc.contributor.authorMartínez-Fresneda, Lucía
dc.contributor.authorO’Brien, Emma
dc.contributor.authorVelázquez, Rosario
dc.contributor.authorToledano-Díaz, Adolfo
dc.contributor.authorMartínez-Cáceres, Carlos M.
dc.contributor.authorTesfaye, Dawit
dc.contributor.authorSchellander, Karl
dc.contributor.authorGarcía-Vázquez, Francisco A.
dc.contributor.authorSantiago-Moreno, Julian
dc.date.accessioned2019-11-21T14:42:47Z
dc.date.available2019-11-21T14:42:47Z
dc.date.issued11.06.2019
dc.identifier.urihttp://hdl.handle.net/20.500.11811/1402
dc.description.abstractThe aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with sperm head area and seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout the year which could interfere with spermatogenesis and sperm cryopreservation. Ejaculates, testicular biopsies and blood were collected during the middle and the end of the RS (Middle-RS vs End-RS) during periods of high and low testosterone levels in Merino and Mouflon rams. Fresh and frozen-thawed sperm quality, sperm morphometry, seminiferous tubule morphometry and testicular proliferation markers (proliferating-cell nuclear antigen, Ki67 and GATA-4) were evaluated. Post-thaw sperm viability was higher in the End-RS in Merino (69.9±8.2 vs 41.6±7.3%; P=0.020) and Mouflon (40.9±3.3 vs 24.2±5.0%; P=0.008). Mouflons had larger sperm head area at the End-RS (38.3±0.2 vs 34.3±0.1 μm2; P=0.029), whereas there was no difference between Merino groups (35.7±0.5 vs 34.8±1.0 μm2). Seminiferous tubule morphometry and proliferation markers showed higher levels of germinal epithelium proliferation in the Middle-RS of both species. In conclusion, sperm freezability is affected during the RS in domestic and wild rams which could be correlated with changes that occur during spermatogenesis since there is an effect of season on cell proliferation in the testis.de
dc.format.extent38
dc.language.isoeng
dc.rightsIn Copyright
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectcryopreservation
dc.subjecttestis
dc.subjecttestosterone
dc.subjectSertoli cell
dc.subjectspermatogenesis
dc.subjectspermatozoa
dc.subject.ddc630 Landwirtschaft, Veterinärmedizin
dc.titleSeasonal variations of sperm freezability associated with testicular germinal epithelium changes in domestic (Ovis aries) and wild (Ovis musimon) rams
dc.typeWissenschaftlicher Artikel
dc.publisher.nameCSIRO
dc.publisher.locationCollingwood
dc.rights.accessRightsopenAccess
dcterms.bibliographicCitation.volume31
dcterms.bibliographicCitation.issue10
dcterms.bibliographicCitation.pagestart1545
dcterms.bibliographicCitation.pageend1557
dc.relation.doihttps://dx.doi.org/10.1071/RD18511
dcterms.bibliographicCitation.journaltitleReproduction, fertility and development
ulbbn.pubtypeZweitveröffentlichung


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