Schmitz, Ronja: Development of root phenotyping methods and QTL analysis of root traits in grapevine mapping populations. - Bonn, 2023. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
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author = {{Ronja Schmitz}},
title = {Development of root phenotyping methods and QTL analysis of root traits in grapevine mapping populations},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2023,
month = jan,

note = {Facing climatic change and upcoming extreme weather events require the adaptation of agricultural crops to conditions like extended drought periods in the future. Therefore, the incorporation of promising morphological and physiological qualities is necessary in crop breeding strategies. Regarding heat and drought, plant roots play an essential role for adequate water uptake and efficient exploitation of water resources in the soils. As plant roots are crucial for crop performance and yield, investigation of phenotypic root traits is a promising approach to promote breeding efforts. However, phenotyping of root systems has proven to be extremely challenging due to their hidden nature. In this study, grapevine adventitious root development from grapevine woody cuttings was investigated by three different phenotyping methods to identify QTL regions linked to grapevine adventitious root formation and root system characteristics.
The first attempt of classifying early adventitious root formation on woody cuttings grown in perlite substrate resulted in the identification of four QTLs. To further investigate sub-traits of root formation, image-based analysis was envisaged. Therefore, a root phenotyping system based on rhizotrons suitable for high throughput root architecture screenings of mapping populations was developed and evaluated. The utilization of rhizotrons for root phenotyping provided a data rich basis for QTL mapping of two mapping populations and revealed 18 QTLs in total for root related traits and four QTLs for leaf area. Thirdly, certain genotypes of a grapevine mapping population grown in the field were analysed regarding root system characteristics resulting in four identified QTLs in total; three QTLs associated with root system characteristics and one QTL associated with shoot biomass. Finally, the three root system phenotyping methods were compared regarding their performance by examining the individual throughput potential, time and cost effort, number of measurable traits, as well as number and quality of resulting QTL regions.
This study describes the development and analysis of phenotyping methods for grapevine root systems. It contributes to the identification of potential genomic regions and candidate genes involved in the regulation of adventitious root formation providing new insights relevant for breeding purposes and breeding research of grapevine rootstocks.},

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