Ponce-Barajas, Patricio: Expression analysis of homeobox gene family in bovine pre- and post-implantative developmental stages. - Bonn, 2006. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5N-08122
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5N-08122,
author = {{Patricio Ponce-Barajas}},
title = {Expression analysis of homeobox gene family in bovine pre- and post-implantative developmental stages},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2006,
note = {Homeobox (hox) genes encode transcription factors which regulate the expression of several target genes encoding essential proteins for cell differentiation and proliferation. Thus, hox-genes have long been recognized as master regulators of the mammalian development. It was the objective of the current study to investigate the expression profile of hox-gene family members in pre- and postimplantation developmental stages of bovine embryos. For this, EST´s derived from Cdx1, Cdx2, HoxA7, HoxB6, HoxB7, HoxB9, HoxC9, HoxD1, and HoxD4 were sequenced. The quantitative real-time PCR was applied to determine the expression profiling of Cdx1, Cdx2, HoxB7, HoxB9, HoxC9, HoxD1, and HoxD4. Thus, triplicate pools (each containing 10) of immature oocytes, matured oocytes, 2-cell, 4-cell, 8-cell, 16-cell, morula and blastocysts were analysed. Moreover, transcripts were quantified in embryos of day 21, 25, 32, 39. The highest relative abundance was observed for Cdx1, Cdx2, HoxB7, HoxB9, and HoxC9 at the immature oocyte stage and expression was down regulated in the later developmental stages, except for HoxD1 and HoxD4 transcripts, which were abundant at higher level in mature oocytes. Transcripts of Cdx2, HoxB7, HoxB9, and HoxC9 were found to progressively increase after 16 cells stage until blastocyst stage. The Cdx2 transcript was found to have abundant level after implantation, which may support its potential role in embryo implantation. The relative abundance of Cdx1 was found to progressively increase at day 21, 25, 32, and 39, similarly as HoxB7. The expression profile of HoxB9 and HoxC9 genes seems to be similar after implantation, both showed the lowest relative abundance at day 25 and the highest at day 32, declining at day 39. HoxD1 and HoxD4 showed the lowest relative mRNA abundance at day 21, increasing at day 32. At day 39, HoxD1 appeared declined and HoxD4 was increased. In conclusion, the present study has shown that Hox-genes are active transcripts in bovine preimplantation and postimplantation stages, supporting their potential involvement in the early embryonic development as nuclear transcription regulators.},
url = {http://hdl.handle.net/20.500.11811/2378}

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