Phatsara, Chirawath: Analyses of immune competence traits and their association with candidate genes in pigs. - Bonn, 2007. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc:
author = {{Chirawath Phatsara}},
title = {Analyses of immune competence traits and their association with candidate genes in pigs},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2007,
volume = 141,
note = {The present study was carried out to investigate the immune competences of a backcross DUMI population. Complement activity via classical and alternative pathways were determined using haemolytic activity assay. Antibodies response to Mycoplasma hyopneumoniae, Tetanus toxoid and PRRS virus were determined using an Enzyme-Linked Immunosorbent Assay (ELISA). Moreover, complement component C3c and Haptoglobin (Hp), the important parameters of animal immune response were also determined. The parameters were further utilized as phenotypes for the linkage mapping to detect quantitative trait loci (QTL). Microsatellite genotyping was employed to detect the QTL of the immune traits. A total of 220 backcross animals were used for QTL analysis. Seventy-four microsatellites from 18 autosomes of Sus scrofa have been used for QTL mapping. Forty-two significant and twenty-four highly significant QTL could be detected for all immune traits. Most QTL were detected on SSC3, SSC16, and SSC18 (nine significant F-values on each chromosome). No significant F-value was detected on SSC12 and SSC13. Highly significant QTL could be detected for antibody response to Mycoplasma, Tetanus and PRRS vaccination, C3c and Hp concentration. For AH50 and CH50, twenty-two significant and nine highly significant QTL could be detected by using the program QTL express. Mannose-binding lectin (MBL) genes were proposed as a candidate gene in this study. Two porcine genes MBL1 and MBL2 were investigated. A phylogenetic study revealed that the porcine MBL genes had higher identities to bovine rather than primate and rodent sequences. Both genes were assigned to chromosome 14 by the radiation hybrid panel and linkage mapping. Both MBL genes were highly expressed in liver. MBL1 was also found to be expressed in lung, testis and brain, while low expression of MBL2 was detected in testis and kidney. New single nucleotide polymorphisms (SNP) of the porcine MBL2 gene were found and genotyped in an experimental F2 pig population together with a previously reported SNP of MBL1. MBL1 genotypes differed in C3c serum concentration, i.e. in vivo complement activity, at p<0.1. Correspondingly, linkage analysis revealed a QTL for C3c serum level close to the position of the MBL genes. The study thus promotes the porcine MBL genes as functional and positional candidate genes for complement activity.},
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