Ghanem Osman Mohamed, Nasser: Molecular genetic analysis of bovine oocytes with different developmental potentials. - Bonn, 2009. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc:
author = {{Nasser Ghanem Osman Mohamed}},
title = {Molecular genetic analysis of bovine oocytes with different developmental potentials},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2009,
month = jun,

volume = 144,
note = {Poor oocyte developmental potential contributes to reduced fertility in livestock species and hampers the application of biotechnology techniques in cattle industry that could be used for spreading certain genotypes with production advantages. However, the molecular mechanisms regulating oocyte developmental potential are generally poorly understood. Therefore, the objective of this study was to identify differentially regulated genes in oocytes with different developmental potential that could be associated with their competence. For this, oocytes were selected based on phase of follicular turnover (growth vs. dominance) and BCB staining (BCB+ vs. BCB-) as two independent models for screening of oocyte competence. For each model, six pools of oocytes were used for mRNA isolation and subsequent RNA amplification. A custom-made cDNA array with ~ 2000 clones was used to compare the gene expression profiles of competent versus incompetent oocytes of each model. The Significance Analysis of Microarray (SAM) has been used for data generation. A total of 51 and 185 genes to be differentially expressed have been identified between the oocytes derived from growth compared to dominance follicular phase and BCB+ compared to BCB- ones, respectively. Based on biological process annotation, genes involved in protein biosynthesis as structural constituent of ribosome (RPL24, ARL6IP, RPS14 and RPS15), translation elongation factor activity (EEF1A1), chromosome organization and biogenesis (H2AFZ) and signal transduction (GNB2L1) were commonly up-regulated in competent compared to incompetent oocytes of both models. On the other hand, incompetent oocytes from both models were enriched with transcripts regulating transcription (PTTG1) and growth factor activity (BMP 15). Quantitative real-time PCR has confirmed the relative abundance of 8 out of 10 and 9 out of 10 genes to be in accordance with microarray analysis for follicular phase and BCB staining models, respectively. Overall, this study provides a genome-wide expression profiling of genes that could be associated with developmental competence of bovine oocytes. However, further functional investigations based on this data could help to define the exact key regulatory genes controlling oocyte quality that could be considered as good biomarkers.},
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