Browsing by Author "Wachten, Dagmar"

Now showing items 1-20 of 24

    • Action of zona pellucida glycoproteins in mouse and human sperm 

      Balbach, Melanie (2017-08-22)
      For fertilization, sperm have to penetrate the oocyte’s vestment, the zona pellucida (ZP). In mouse and human, the ZP consists of three or four different ZP glycoproteins, respectively. Binding of sperm to ZP glycoproteins ...

    • Analyzing the role of a truncated Adenylyl Cyclase 3 isoform in brown adipose tissue 

      Vagliano, Lara-Marie (2025-01-29)
      Obesity has become one of the main public health issues in the 21<sup>st</sup> century. Targeting brown adipose tissue (BAT) activity, which is physiologically induced by cold temperatures, has been recognized as a potential ...

    • Cilia-dependent signaling pathways controlling adipose tissue plasticity 

      Winnerling, Nora Louise (2022-11-15)
      Primary cilia serve as cellular antennae that detect extracellular signals and transduce this information into an intracellular response. Dysfunction of primary cilia leads to a range of severe diseases, i.e., the ...

    • Cold-induced expression of a truncated adenylyl cyclase 3 acts as rheostat to brown fat function 

      Khani, Sajjad; Topel, Hande; Kardinal, Ronja; Tavanez, Ana Rita; Josephrajan, Ajeetha; Larsen, Bjørk Ditlev Marcher; Gaudry, Michael James; Leyendecker, Philipp; Meincke Egedal, Nadia; Güller, Aylin Seren; Stanic, Natasa; Ruppert, Phillip M. M.; Gaziano, Isabella; Hansmeier, Nils Rouven; Schmidt, Elena; Klemm, Paul; Vagliano, Lara-Marie; Stahl, Rainer; Duthie, Fraser; Krause, Jens-Henning; Bici, Ana; Engelhard, Christoph Andreas; Gohlke, Sabrina; Frommolt, Peter; Gnad, Thorsten; Rada-Iglesias, Alvaro; Pradas-Juni, Marta; Schulz, Tim Julius; Wunderlich, Frank Thomas; Pfeifer, Alexander; Bartelt, Alexander; Jastroch, Martin; Wachten, Dagmar; Kornfeld, Jan-Wilhelm (2024-04-29)
      Promoting brown adipose tissue (BAT) activity innovatively targetsobesity and metabolic disease. While thermogenic activation of BAT is wellunderstood, the rheostatic regulation of BAT to avoid excessive energy dissipation ...

    • The crosstalk of macrophages and the adipose tissue 

      Yüksel, Seniz (2026-04-07)
      The primary cilium is a highly conserved sensory organelle found on most mammalian cells, which senses environmental signals and transduces this information into a cellular response. Within the adipocyte linage, only the ...

    • The Effects of Maternal Obesity on the Innate Immune System: Developmental Programming of Kupffer Cells 

      Balzer, Nóra Réka (2023-03-29)
      The global epidemic of obesity has led to an increasing number of obese women in childbearing age. It is now understood that maternal obesity has harmful consequences on fetal and adult health. The underlying mechanism of ...

    • Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors 

      Klausen, Christina; Kaiser, Fabian; Stüven, Birthe; Hansen, Jan N.; Wachten, Dagmar (2019-11-14)
      The second messenger 3´, 5´-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil ...

    • How to control cyclic nucleotide signaling by light 

      Jansen, Vera; Jikeli, Jan F.; Wachten, Dagmar (2017-03-10)
      Optogenetics allows to non-invasively manipulate cellular functions with spatio-temporal precision by combining genetic engineering with the control of protein function by light. Since the discovery of channelrhodopsin has ...

    • Ingestion of micro- and nanoplastic perturbs tissue homeostasis and macrophage core functions 

      Makdissi, Nikola (2025-11-07)
      Plastic pollution has emerged as a global environmental crisis of unprecedented scale. Micro- and nanoplastic (MNP) pervade ecosystems, resulting in persistent exposure for all living organisms, including humans. MNP have ...

    • Investigating the GBA1-dependent regulation of GBA2 activity in Gaucher disease 

      Schonauer, Sophie (2017-08-01)
      Glycosphingolipids (GSLs) are a large and heterogeneous class of lipids, whose function is equally versatile and complex. They consist of a membrane anchor, ceramide, and different kinds of sugar head groups. Glucosylceramide ...

    • Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue 

      Silva Ribeiro, Dalila Juliana; Yüksel, Seniz; Dolf, Andreas; Wachten, Dagmar (2023-08-29)
      Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and remodeling. Macrophage function in WAT is determined by ontogeny and the local ...

    • Kupffer cell programming by maternal obesity triggers fatty liver disease 

      Huang, Hoa; Balzer, Nora R.; Seep, Lea; Splichalova, Iva; Blank-Stein, Nelli; Viola, Maria Francesca; Taveras, Eliana Franco; Acil, Kerim; Fink, Diana; Petrovic, Franzisca; Makdissi, Nikola; Bayar, Seyhmus; Mauel, Katharina; Radwaniak, Carolin; Zurkovic, Jelena; Kayvanjoo, Amir H.; Wunderling, Klaus; Jessen, Malin; Yaghmour, Mohamed H.; Kenner, Lukas; Ulas, Thomas; Grein, Stephan; Schultze, Joachim L.; Scott, Charlotte L.; Guilliams, Martin; Liu, Zhaoyuan; Ginhoux, Florent; Beyer, Marc D.; Thiele, Christoph; Meissner, Felix; Hasenauer, Jan; Wachten, Dagmar; Mass, Elvira (2025-06-18)
      Kupffer cells (KCs) are tissue-resident macrophages that colonize the liver early during embryogenesis. Upon liver colonization, KCs rapidly acquire a tissue-specific transcriptional signature, mature alongside the developing ...

    • Organization and function of signaling molecules in sperm 

      Hamzeh, Hussein (2017-09-15)
      Successful fertilization depends on the ability of sperm to locate the egg. Sperm from different species rely on diverse signaling components to gather chemical and physical cues and transduce them into a behavioral swimming ...

    • Potent optogenetic regulation of gene expression in mammalian cells for bioproduction and basic research 

      Gebel, Jeannette; Ciglieri, Elisa; Stahl, Rainer; Duthie, Fraser; Frechen, Fabian; Möglich, Andreas; Müller-Hartmann, Herbert; Schmidt, Hanns-Martin; Wachten, Dagmar (2025-06-30)
      Precise temporal and spatial control of gene expression greatly benefits the study of specific cellular circuits and activities. Compared to chemical inducers, light-dependent control of gene expression by optogenetics ...

    • Primary cilia and their effects on immune cell functions and metabolism: a model 

      Picon-Galindo, Ernesto; Latz, Eicke; Wachten, Dagmar (2022-05)
      Primary cilia are hair-like protrusions of the plasma membrane that function as cellular antennae and are present on most cells in the human body. Primary cilia dysfunction leads to severe diseases, commonly termed ...

    • Primary cilia signalling at a glance 

      Wachten, Dagmar; Christensen, Søren Tvorup (2025-10-29)
      The primary cilium is a solitary, microtubule-based organelle present on most vertebrate cells, where it functions as a central hub for sensing and transducing extracellular signals. This Cell Science at a Glance article ...

    • The primary cilium is a unique lipid microdomain engineered to transduce extracellular cues 

      Kaiser, Fabian (2022-04-06)
      Cilia are membrane protrusions located at the surface of almost every vertebrate cell. Cilia come in two different flavors: motile cilia, which generate a fluid flow or propel cells for active movement, and immotile, also ...

    • Renal tissue-resident macrophages promote cystogenesis in early polycystic kidney disease 

      Karl, Rudolfo; Ashraf, Arsila Palliyulla Kariat; Viola, Maria Francesca; Hopp, Katharina; Mass, Elvira; Wachten, Dagmar (2025-08-26)
      Autosomal-dominant polycystic kidney disease (ADPKD) is a ciliopathy characterized bymutations in <em>PKD1</em> or <em>PKD2</em>, which drive cystogenesis in renal epithelial cells. Immune cells, particularly macrophages, ...

    • The role of GBA2 in controlling locomotor activity 

      Woeste, Marina Amelie (2018-12-13)
      Glycosphingolipids are major constituents of cellular membranes, fulfilling both structural and functional roles. Modifications of the basic glycosphingolipid glucosylceramide (GlcCer) result in numerous complex glycosylated ...

    • The role of primary cilia in adipose tissue 

      Sieckmann, Maria Katharina (2025-07-04)
      The primary cilium serves as a cellular antenna, transducing extracellular stimuli into an intracellular response. In white adipose tissue (WAT), adipocyte precursor cells (APCs) but not mature adipocytes display a primary ...