Pirayesh, Niloufar: The CAS protein and Ca2+ signaling in the chloroplast of Arabidopsis thaliana. - Bonn, 2023. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5-70090
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5-70090,
author = {{Niloufar Pirayesh}},
title = {The CAS protein and Ca2+ signaling in the chloroplast of Arabidopsis thaliana},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2023,
month = mar,

note = {There has been evidence that CAS forms a complex in the chloroplast of Chlamydomonas with PSI, CYTb6/f, and other proteins like PGRL1 and ANR1. However, in A. thaliana, such a CAS complex has not been shown yet. Therefore, one of the purposes of this study was to investigate whether CAS can also build a protein complex in the chloroplast of A. thaliana. On the other hand, to better understand the role of CAS in A. thaliana, growth phenotype and photosynthetic activity were investigated in a cas-knockout (casko) A. thaliana mutant line compared to wild-type plants (WT).
CAS was also investigated as part of the Ca2+ signaling pathway in response to salt, mannitol, and oxidative stress. Using WT and casko mutant plants expressing the Ca2+ reporter AEQ, targeted to the cytosol, stroma, and thylakoid lumen, changes in [Ca2+] in response to these stimuli was examined. Additionally, this study has focused on identifying the potential sources of Ca2+ that may contribute to the change in [Ca2+].
Furthermore, we also sought to determine the correct cellular localization of CML36, one of the 50 CMLs found in the A. thaliana, by using the full-length YFP-tag and the self-assembly GFP (saGFP) system in leaf tissue and isolated protoplasts and using laser confocal fluorescence microscopy.},

url = {https://hdl.handle.net/20.500.11811/10697}

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