Development of capillary electrophoresis-based assays for nucleoside and nucleotide metabolizing enzymes

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Author
Iqbal, Jamshed
Type of Scholarly Publication
Dissertation
Date of Exam
27.10.2005
Date of Publication
2005
Advisor
Müller, Christa E.
Co-Referee
Neugebauer, Michael
Involved Institutions
Rheinische Friedrich-Wilhelms-Universität Bonn
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Abstract
The aim of the present work was to develop and optimise capillary electrophoresis-based methods for the assaying of nucleoside and nucleotide metabolizing enzymes.
More specifically the aim included:
  • Developing and optimizing a micellar electrokinetic capillary chromatographic method for the separation of nucleotides and nucleosides.
  • Developing, optimizing and validating a capillary zone electrophoresis method for the separation of nucleotides and their enzymatic degradation products and application of the method to study nucleotide metabolism.
  • Developing and validating an electrophoretically mediated microanalysis with partial-filling technique for the in-line monitoring of adenosine kinase reactions.
  • Developing, optimizing and validating at-capillary-inlet enzymatic microreaction methods for the characterization of ecto-nucleoside triphosphate diphosphohydrolases and automation of the analytical process.
  • Developing and validating an at-capillary-inlet reaction method to study the extracellular nucleotide metabolism of living cells.
Subjects
Pharmazie, Capillary electrophoresis, adenosine kinase assay, thymidine kinase assay, electrophoretically mediated microanalysis, NTPDase assay, kinetics, Michaelis-Menten analysis, in-capillary enzymatic reaction, CE inhibition assay
Classification (DDC)
540 Chemie
610 Medizin, Gesundheit
Iqbal, Jamshed: Development of capillary electrophoresis-based assays for nucleoside and nucleotide metabolizing enzymes. - Bonn, 2005. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5N-06431
@phdthesis{handle:20.500.11811/2328,
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5N-06431,
author = {{Jamshed Iqbal}},
title = {Development of capillary electrophoresis-based assays for nucleoside and nucleotide metabolizing enzymes},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2005,
note = {The aim of the present work was to develop and optimise capillary electrophoresis-based methods for the assaying of nucleoside and nucleotide metabolizing enzymes.
More specifically the aim included:
  • Developing and optimizing a micellar electrokinetic capillary chromatographic method for the separation of nucleotides and nucleosides.
  • Developing, optimizing and validating a capillary zone electrophoresis method for the separation of nucleotides and their enzymatic degradation products and application of the method to study nucleotide metabolism.
  • Developing and validating an electrophoretically mediated microanalysis with partial-filling technique for the in-line monitoring of adenosine kinase reactions.
  • Developing, optimizing and validating at-capillary-inlet enzymatic microreaction methods for the characterization of ecto-nucleoside triphosphate diphosphohydrolases and automation of the analytical process.
  • Developing and validating an at-capillary-inlet reaction method to study the extracellular nucleotide metabolism of living cells.
},
url = {https://hdl.handle.net/20.500.11811/2328}
}

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