Role of Pathogenic Mediators in murine Arthritis and Levels of Serum Soluble CD21 and CD23 in autoimmune patients

Abstract

Importance of the nervous system in immune regulation is clearly suggested by innervations of lymphoid tissues by sympathetic nerve fibers. We determined the role of sympathetic, parasympathetic and peripheral nervous system in K/BxN serum-induced arthritis.<br /> We also demonstrate the role of MIF in K/BxN serum-induced arthritis. Recombinant MIF is an inducer of monocyte/macrophage TNF α and MIF also induces or enhances other aspects of monocytes/macrophage activity, including interferon-γ-induced nitric oxide production, intracellular killing and phagocytic function.<br /> Then, we determined the role of MMP-13 in K/BxN serum-induced arthritis which degrade collagen type I, II and III. But preferentially MMP-13 cleave collagen type II which is predominantly express on articular cartilage and also high expression of MMP-13 has been found in synovial tissue, synovial membrane and articular cartilage of RA patients as compared to healthy patients. <br /> Then, we determined the role of redox-reactions in K/BxN serum-induced arthritis. For this experiment we used phytol which increases the oxidative burst in vivo by activation of NADPH complex.<br /> Then, we measured the levels of sCD21 and sCD23 in various autoimmune patients’ sera/plasma samples and compared with healthy donor. We used Antiphospholipid syndrome patient’s serum, diabetes patient’s serum, autoimmune lymphoproliferative syndrome (ALPS) and Juvenile arthritis subtype plasma samples and measured sCD21 and sCD23 levels by ELISA and compared with their age matched healthy donor.