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Cell-cell adhesion mediated by mobile receptor-ligand pairs
a biomimetic study

dc.contributor.advisorMerkel, Rudolf
dc.contributor.authorFenz, Susanne Franziska
dc.date.accessioned2020-04-13T23:47:44Z
dc.date.available2020-04-13T23:47:44Z
dc.date.issued07.05.2009
dc.identifier.urihttp://hdl.handle.net/20.500.11811/4070
dc.description.abstractThis thesis aims at a quantitative understanding of the physical processes in cell-cell adhesion caused by mobile cell adhesion molecules. To unequivocally separate physical processes from active biological cell response, a biomimetic model system was developed. It consisted of a giant unilamellar vesicle adhering via specific ligand-receptor interactions to a solid supported lipid bilayer. Adhesion was mediated by either biotin-neutravidin (an avidin analogue) or the extracellular domains of the homophilic cell adhesion molecule E-cadherin. A microscope was modified for simultaneous application of fluorescence microscopy and microinterferometry.
In membrane adhesion mediated by both binding pairs in high concentration the final equilibrium was characterized with respect to membrane distance, tension, and adhesion energy density with the help of microinterferometry. Fluorescently labelled neutravidin was used to infer the receptor distribution and concentration beneath the adhering vesicle. Moreover, the fluidity of the SLB and the diffusion of the receptors was studied during vesicle adhesion by continuous photobleaching. Dynamics of vesicle adhesion (nucleation, growth and saturation) were analyzed for a broad range of receptor-ligand concentrations for strong binding and at high concentrations for weak binding.
dc.language.isoeng
dc.rightsIn Copyright
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectZelladhäsion
dc.subjectModellsystem
dc.subjectMembran
dc.subjectVesikel
dc.subjectMikroskopie
dc.subjectReflektions-Interferenz-Kontrast-Mikroskopie
dc.subjectFluoreszenzmikroskopie
dc.subjectDiffusion
dc.subjectPhotobleichen
dc.subjectBiotin
dc.subjectNeutravidin
dc.subjectE-Cadherin
dc.subjectcell adhesion
dc.subjectmodel system
dc.subjectmembrane
dc.subjectvesicle
dc.subjectmicroscopy
dc.subjectreflection interference contrast microscopy
dc.subjectfluorescence microscopy
dc.subjectphotobleaching
dc.subject.ddc540 Chemie
dc.subject.ddc570 Biowissenschaften, Biologie
dc.titleCell-cell adhesion mediated by mobile receptor-ligand pairs
dc.title.alternativea biomimetic study
dc.typeDissertation oder Habilitation
dc.publisher.nameUniversitäts- und Landesbibliothek Bonn
dc.publisher.locationBonn
dc.rights.accessRightsopenAccess
dc.identifier.urnhttps://nbn-resolving.org/urn:nbn:de:hbz:5N-17420
ulbbn.pubtypeErstveröffentlichung
ulbbnediss.affiliation.nameRheinische Friedrich-Wilhelms-Universität Bonn
ulbbnediss.affiliation.locationBonn
ulbbnediss.thesis.levelDissertation
ulbbnediss.dissID1742
ulbbnediss.date.accepted2009-03-20
ulbbnediss.fakultaetMathematisch-Naturwissenschaftliche Fakultät
dc.contributor.coRefereeKubitscheck, Ulrich


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