The Liprin-α protein familycommon and diverging properties
The Liprin-α protein family
common and diverging properties
dc.contributor.advisor | Schoch, Susanne | |
dc.contributor.author | Zürner, Magdalena | |
dc.date.accessioned | 2020-04-15T21:16:57Z | |
dc.date.available | 2020-04-15T21:16:57Z | |
dc.date.issued | 21.12.2010 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11811/4699 | |
dc.description.abstract | The Liprin-α family of scaffolding proteins is highly evolutionary conserved. While one homologue is present in invertebrates, it has diversified into four isoforms in mammals. In invertebrates, Liprin-α plays a crucial role in synapse assembly and maintenance as well as in synaptic vesicle trafficking. Little is known about the functional role of the four Liprin-α isoforms in the mammalian brain. The aim of this study was to examine the common and diverging properties of the four Liprin-α isoforms in order to gain insight into their role at the mammalian synapse. A comparative characterization of the structure of the human and mouse Liprin-α genes and their regulation by alternative splicing showed that even though the genomic organization of the four isoforms is very similar, Liprins-α can be modified differentially in a developmental manner. To study the spatiotemporal expression pattern of the four Liprin-α isoforms we generated isoform-specific peptide antibodies. All four Liprin-α proteins are expressed in most neuronal populations of the brain, but each Liprin-α protein is characterized by a distinct expression profile. In particular, the spatiotemporal expression pattern of Liprin-α1 stands out. Expression of the most abundant isoforms, Liprin-2α and -3α, and the more weakly expressed Liprin-4α is restricted to the brain and increases during development. In contrast, Liprin-1α is expressed in all tissues tested, is present in glia, and has the highest expression level during development. The overlapping and distinct regional and subcellular localization of the Liprins-α indicates common as well as diverging functional roles. These results support the hypothesis that Liprin-1α might play a role in organizing protein complexes that are involved in cell migration. Liprins-α are hypothesized to act as scaffolding proteins at the active zone. To gain a better understanding of the role of Liprin-α2 at the presynapse we analyzed its dynamics. We found that Liprin-α2 has a relatively slow turn-over which suggests that Liprin-α2 functions as a stably integrated scaffolding protein, thereby adding to the tenacity of the active zone. For further investigations into the role of Liprins-α we identified shRNAs to knock down single isoforms as well as a point mutation to disrupt Liprin-α-RIMα interactions. In summary, this study provides a thorough characterization of the Liprin-α family, and indicates a role in cell migration during brain development for Liprin-α1 and a role in synapse tenacity for Liprin-α2. | |
dc.language.iso | eng | |
dc.rights | In Copyright | |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | |
dc.subject | Aktive Zone | |
dc.subject | Synapse | |
dc.subject | Liprin | |
dc.subject | Active Zone | |
dc.subject.ddc | 570 Biowissenschaften, Biologie | |
dc.title | The Liprin-α protein family | |
dc.title.alternative | common and diverging properties | |
dc.type | Dissertation oder Habilitation | |
dc.publisher.name | Universitäts- und Landesbibliothek Bonn | |
dc.publisher.location | Bonn | |
dc.rights.accessRights | openAccess | |
dc.identifier.urn | https://nbn-resolving.org/urn:nbn:de:hbz:5N-23750 | |
ulbbn.pubtype | Erstveröffentlichung | |
ulbbnediss.affiliation.name | Rheinische Friedrich-Wilhelms-Universität Bonn | |
ulbbnediss.affiliation.location | Bonn | |
ulbbnediss.thesis.level | Dissertation | |
ulbbnediss.dissID | 2375 | |
ulbbnediss.date.accepted | 19.11.2010 | |
ulbbnediss.fakultaet | Mathematisch-Naturwissenschaftliche Fakultät | |
dc.contributor.coReferee | Haas, Albert |
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