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L-Proline and Glutamatergic Neurotransmission
Clarifying the Modulatory Role of Neuronal L-Proline Transporter

dc.contributor.advisorKostenis, Evi
dc.contributor.authorSchulz, Daniel
dc.date.accessioned2020-04-17T22:30:19Z
dc.date.available2020-04-17T22:30:19Z
dc.date.issued20.06.2012
dc.identifier.urihttp://hdl.handle.net/20.500.11811/5320
dc.description.abstractThe neuronal high affinity L-proline transporter (PROT) is a putative neurotransmitter transporter whose contribution to neurotransmission is still unknown. PROT is expressed exclusively in brain by subpopulations of glutamatergic neurons and is assumed to conduct the reuptake of L-proline, which is released upon depolarization. Since to date no specific high-affinity receptor for L-proline has been discovered, the amino acid has been suggested to play a role regulating glutamatergic neurotransmission. To uncover the in vivo modulatory function of PROT, a mouse strain lacking functional PROT was generated and confirmed. The analysis of these PROT-knockout mice provided new insights into the modulatory functional roles of this transporter. Biochemical alterations within the central nervous system of PROT lacking mice were identified. Thus, PROT-deficient mice exhibit increased expression levels of N-methyl-D-aspartic acid (NMDA), α-amino-3-hydroxy-5 methylisoxazolepropionic acid (AMPA) and kainate (KA) receptor subunits. Furthermore, levels of the dopamine receptor D2 (DRD2) as well as levels of the dopamine synthesizing enzyme tyrosine hydroxylase (TH) are elevated. Behavioral analyses of PROT-knockout mice unveiled decreased locomotor activity, increased anxiety like behavior, and better learning abilities compared to wild-type littermates. The changes in behavioral profiles of analyzed PROT-deficient animals are in line with the observed biochemical adaptive alterations. Collectively, the presented studies indicate that the functional role of PROT within neurotransmission is primarily the modulatory fine tuning of glutamatergic signaling, by controlling the levels of L-proline in synaptic clefts.
dc.language.isoeng
dc.rightsIn Copyright
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subject.ddc500 Naturwissenschaften
dc.titleL-Proline and Glutamatergic Neurotransmission
dc.title.alternativeClarifying the Modulatory Role of Neuronal L-Proline Transporter
dc.typeDissertation oder Habilitation
dc.publisher.nameUniversitäts- und Landesbibliothek Bonn
dc.publisher.locationBonn
dc.rights.accessRightsopenAccess
dc.identifier.urnhttps://nbn-resolving.org/urn:nbn:de:hbz:5n-28808
ulbbn.pubtypeErstveröffentlichung
ulbbnediss.affiliation.nameRheinische Friedrich-Wilhelms-Universität Bonn
ulbbnediss.affiliation.locationBonn
ulbbnediss.thesis.levelDissertation
ulbbnediss.dissID2880
ulbbnediss.date.accepted2012-03-26
ulbbnediss.fakultaetMathematisch-Naturwissenschaftliche Fakultät
dc.contributor.coRefereeMohr, Klaus


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