Klein, Katharina M.: Generation and Characterisation of an HRP-3-deficient Mouse Model. - Bonn, 2013. - Dissertation, Rheinische Friedrich-Wilhelms-Universität Bonn.
Online-Ausgabe in bonndoc: https://nbn-resolving.org/urn:nbn:de:hbz:5n-33350
@phdthesis{handle:20.500.11811/5756,
urn: https://nbn-resolving.org/urn:nbn:de:hbz:5n-33350,
author = {{Katharina M. Klein}},
title = {Generation and Characterisation of an HRP-3-deficient Mouse Model},
school = {Rheinische Friedrich-Wilhelms-Universität Bonn},
year = 2013,
month = sep,

note = {The hepatoma-derived growth factor-related protein-3 (HRP-3) is a growth factor of the HDGF protein family with neurotrophic activity. Predominantly expressed in the brain, it was shown to bind and stabilise microtubules. A function during brain development is speculated in the literature.
Targeting of the HRP-3 locus has been hitherto unsuccessful. In this work, a non-conditional targeting vector targeting exon 2 of HRP-3 was designed and a mutant mouse line was successfully created. Mice were viable and fertile and lived up to the age of ten months without any obvious abnormalities. A single case of a six-month-old mouse with small lesions in the cerebral cortex was found, but due to the absence of older animals, these results were not reproduced.
Using this mouse model, it was discovered that HRP-3 is indeed predominantly expressed in the brain, but also, in contrast to findings in contemporary literature, to lesser extent in a range of other tissues and specific cell types. These include podocytes in the kidney and heart muscle cells. It can thus be supposed that the function of HRP-3 is not only confined to the brain, but also extends to other organs. Additionally, it was also shown that expression of HRP-3 within the brain extends to more cell types than previously reported.
Furthermore, HRP-3-deficient animals exhibited a hyperplasia of stomach mucosa and submucosa, resulting in extreme stomach folds. Since it was shown here that HRP-3 is expressed in several cell types in the stomach, a phenotype is conceivable. A reason for these foldings was not yet found and remains to be elucidated. Hyperplasia of the stomach mucosa is characteristic for the human Ménétrier disease. Whether HRP-3-deficient mice resemble such a disease is object for further analyses.
In summary, an HRP-3-deficient mouse model was successfully created and first analyses were able to give new HRP-3 expression data. A more extensive analysis, especially of the stomach abnormalities, is necessary and might provide new insights into the function of HRP-3.},

url = {http://hdl.handle.net/20.500.11811/5756}
}

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