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Locus coeruleus modulates locomotor activity via the medial septum and the diagonal band of Broca

dc.contributor.advisorRemy, Stefan
dc.contributor.authorLudwig, Felix
dc.date.accessioned2020-10-02T10:22:22Z
dc.date.available2020-10-02T10:22:22Z
dc.date.issued02.10.2020
dc.identifier.urihttp://hdl.handle.net/20.500.11811/8640
dc.description.abstractThe locus coeruleus (LC) is positioned in the brainstem and one of the main sources of norepinephrine in the brain. With its projections throughout the whole brain, the LC plays an important role in regulating the general level of arousal and in motivation. The present study aims to identify specific anatomical and functional connectivity patterns between the LC and cell populations in the medial septum diagonal band (MSDB). The direct activation of tyrosine hydroxylase positive neurons in LC has been shown to induce general locomotor arousal and changes in the hippocampal theta activity (Carter et al. 2010). It was shown, that the MSDB is integrated into locomotor activity and controls hippocampal locomotion dependent theta oscillation (Fuhrmann et al. 2015). In this study LC afferent fibers in the MSDB get activated optogenetically in order to investigate if noradrenergic input modulates MSDB activity and alters running behavior. Histology of Th-Cre mice with EYFP Injections in the LC confirms projections to the MSDB area. The results of this study indicate that optogenetic stimulation of noradrenergic locus coeruleus afferents in MSDB leads to increased running behavior in terms of running duration and running initiations but not velocity. Follow-up slice preparations of MSDB placed on a multi electrode array can be used to monitor spontaneous in-vivo like network and single unit activity. 15% of these units increase their firing when noradrenergic afferents are stimulated. Also, 30% of all recorded Units are theta modulated, but no effect on theta modulation could be observed. The two dimensional structure of the MEA allows to further distinguish between dorsal and ventral distributed unit populations within the MSDB. Differences in firing frequency and burst probability along the coronal section of the MSDB indicate a heterogeneous LC input with a majority of noradrenalin sensitive cells in the dorsal MS, rather than the DB.en
dc.language.isoeng
dc.rightsIn Copyright
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectLocus Coeruleus
dc.subjectMediales Septum
dc.subjectdiagonales Band von Broca
dc.subjectBasales Vorderhirn
dc.subjectHippocampus
dc.subjectBewegung
dc.subjectErregung
dc.subjectLaufen
dc.subjectMaus
dc.subjectin vivo
dc.subjectin vitro
dc.subjectLaufband
dc.subjectMultielektroden Array
dc.subjectPatch Clamp Methode
dc.subjectHistologie
dc.subjectLokale Feldpotentiale
dc.subjectmedial septum
dc.subjectdiagonal band of Broca
dc.subjectbasal forebrain
dc.subjectmovement
dc.subjectarousal
dc.subjectrunning behavior
dc.subjectmous
dc.subjecttreadmill
dc.subjectmultielectroden array
dc.subjectpatch clamp
dc.subjecthistology
dc.subjectLFP
dc.subject.ddc500 Naturwissenschaften
dc.subject.ddc570 Biowissenschaften, Biologie
dc.subject.ddc610 Medizin, Gesundheit
dc.titleLocus coeruleus modulates locomotor activity via the medial septum and the diagonal band of Broca
dc.typeDissertation oder Habilitation
dc.publisher.nameUniversitäts- und Landesbibliothek Bonn
dc.publisher.locationBonn
dc.rights.accessRightsopenAccess
dc.identifier.urnhttps://nbn-resolving.org/urn:nbn:de:hbz:5-59829
ulbbn.pubtypeErstveröffentlichung
ulbbnediss.affiliation.nameRheinische Friedrich-Wilhelms-Universität Bonn
ulbbnediss.affiliation.locationBonn
ulbbnediss.thesis.levelDissertation
ulbbnediss.dissID5982
ulbbnediss.date.accepted2020-08-18
ulbbnediss.instituteAngegliederte Institute, verbundene wissenschaftliche Einrichtungen : Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
ulbbnediss.fakultaetMathematisch-Naturwissenschaftliche Fakultät
dc.contributor.coRefereeWitke, Walter


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